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The scenario that best exemplifies non-verbal communication is nodding while listening. Nodding is a form of non-verbal cue that signals acknowledgment, understanding, or agreement without the use of spoken words. It serves as a way to engage in a conversation and convey attentiveness, demonstrating that the listener is processing the information being shared.

In contrast, the alternative scenario of saying "I'm not worried" with a shaky voice while staring wide-eyed at a needle does involve verbal communication, albeit with conflicting non-verbal cues. The shaky voice and wide-eyed expression communicate anxiety, which contradicts the verbal message, making it a mixed signal rather than a pure example of non-verbal communication.

Giving a thumbs up does represent a clear form of non-verbal affirmation but is more specific than the broader context of listening and engagement that nodding encompasses. Looking away while speaking may also convey disinterest or discomfort, but it does not actively communicate a positive response like nodding does while listening.